Reducing Vs Non Reducing Sds Page

Separation of low molecular weight oligomers. Nonreducing SDSPAGE

Reducing Vs Non Reducing Sds Page. So in reducing sds, you add bme or another. A reducing agent can break disulfide bonds, and for a majority of proteins, this will not.

Separation of low molecular weight oligomers. Nonreducing SDSPAGE
Separation of low molecular weight oligomers. Nonreducing SDSPAGE

So in reducing sds, you add bme or another. If we had a heterotrimer, we would only see one band. A reducing agent can break disulfide bonds, and for a majority of proteins, this will not.

If we had a heterotrimer, we would only see one band. If we had a heterotrimer, we would only see one band. So in reducing sds, you add bme or another. A reducing agent can break disulfide bonds, and for a majority of proteins, this will not.